Ngwa nke ultrafiltration na nkewa protein na ịdị ọcha

Teknụzụ Ultrafiltration bụ teknụzụ nkewa dị elu na nke dị elu.Ọ nwere njirimara nke usoro dị mfe, uru akụ na ụba dị elu, enweghị mgbanwe mgbanwe nke oge, nnukwu ọnụọgụ nkewa, nchekwa ike, arụmọrụ dị elu, enweghị mmetọ nke abụọ, ọrụ na-aga n'ihu na okpomọkụ ụlọ na ihe ndị ọzọ.Taa, onye njikwa Yang onye si Beijing jụrụ ajụjụ gbasara akụrụngwa ultrafiltration anyị maka ịdị ọcha protein wee jiri teknụzụ anyị gwa ya nke ọma.Ugbu a, onye nchịkọta akụkọ nke Shandong Bona otu ga-ewebata ngwa nke ultrafiltration na protein nkewa na ịdị ọcha.

1. N'ihi na protein desalination, dealcoholization na itinye uche
Ngwa kachasị mkpa nke ultrafiltration na-adị ọcha nke protein bụ desalting na itinye uche.Ultrafiltration usoro ka desalination na ịta na-ji nnukwu ogbe olu, obere ọrụ oge na elu arụmọrụ nke protein mgbake.Usoro ọdịnala nke steepụ mwepu chromatography iji wepụ ihe dị iche iche sitere na protein ejiri teknụzụ ultrafiltration nke ọgbara ọhụrụ dochie ya, nke ghọrọ teknụzụ bụ isi maka mkpochapụ protein, dealcoholization na itinye uche taa.N'ime afọ ndị na-adịbeghị anya, a na-eji teknụzụ ultrafiltration eme ihe n'ọtụtụ ebe na nsị na mgbake nke protein bara uru dị na cheese whey na soybean whey.Lactose na salts na ihe ndị ọzọ dị na protein, yana mkpa n'ezie nke ọma na-emecha desalting, de-alcoholization na ịta nke protein.Ojiji nke teknụzụ ultrafiltration nwekwara ike itinye uche na serospecies immunoglobulins iji gboo mkpa protein n'ezie.

2. N'ihi na protein fractionation
Mpempe akwụkwọ protein na-ezo aka na usoro nkewapụ akụkụ nke protein ọ bụla site na nkebi dịka ọdịiche nke anụ ahụ na nke kemịkal (dị ka ịdị arọ molekụla, ebe isoelectric, hydrophobicity, wdg) nke protein ọ bụla dị na mmiri mmiri nri.Gel chromatography bụ otu n'ime ụzọ ndị a na-ejikarị eme ka akụkụ nke macromolecules biological (karịsịa protein).E jiri ya tụnyere chromatography ọdịnala, teknụzụ nkewa nke ultrafiltration nwere atụmanya dị mma nke itinye n'ọrụ na nbibi na mmepụta mmepụta nke protein na enzymes nwere uru akụ na ụba dị mkpa n'ihi ọnụ ahịa ya dị ala na mmụba dị mfe.Akwa ọcha bụ akụrụngwa dị ọnụ ala iji nweta lysozyme na ovalbumin.N'oge na-adịbeghị anya, a na-ejikarị ultrafiltration kewapụ ovalbumin na lysozyme na akwa ọcha.

3. Mwepụ Endotoxin
Mwepụ Endotoxin bụ otu n'ime ụdị ngwa ngwa nke teknụzụ ultrafiltration na nchacha protein.Usoro mmepụta nke endotoxin dị mgbagwoju anya.N'ime usoro nke ngwa bara uru, n'ihi na protein ọgwụ na-emepụta site na usoro okwu prokaryotic dị mfe ịgwakọta na endotoxin na-emepụta site na mgbidi mgbidi nke nje bacteria na-emepụta, na endotoxin, nke a makwaara dị ka pyrogen, bụ ụdị lipopolysaccharide.Mgbe ịbanye n'ime ahụ mmadụ, ọ nwere ike ịkpata ahụ ọkụ, microcirculation ọgba aghara, endotoxic ujo na mgbaàmà ndị ọzọ.Iji chebe ahụike mmadụ, ọ dị mkpa iji teknụzụ ultrafiltration n'ụzọ zuru ezu wepụ endotoxins.

Ọ bụ ezie na a na-eji teknụzụ ultrafiltration eme ihe na nkewa na ịdị ọcha nke protein, ọ nwekwara oke ụfọdụ.Ọ bụrụ na ịdị arọ molekụla nke ngwaahịa abụọ a ga-ekewa bụ ihe na-erughị ugboro 5, enweghị ike ikewa ya site na ultrafiltration.Ọ bụrụ na ịdị arọ molekụla nke ngwaahịa ahụ erughị 3kD, ọ nweghị ike itinye uche ya site na ultrafiltration, n'ihi na a na-eme ultrafiltration na opekempe ịdị arọ nke akpụkpọ ahụ na 1000 NWML.

Site na mmepe nke ụlọ ọrụ bioengineering na-aga n'ihu, a na-atụ anya ihe ndị dị elu maka ikewapụ na teknụzụ dị ọcha.Ụzọ ọdịnala nke itinye uche agụụ, mmịpụta ihe mgbaze, dialysis, centrifugation, ọdịda mmiri na iwepụ pyrogen adịghị adị iji gboo mkpa mmepụta ọzọ.A ga-eji teknụzụ Ultrafiltration eme ihe n'ihi na ọ bara uru na nkewa protein.